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Protein expression was induced with 2% galactose at 15 °C for 48 h then harvested. All further steps were carried out at 4 °C. Here’s what 5 days on a 3,000-calorie diet may look like. To explore this further we measured mRNA and protein levels of αSyn 3K-GFP with CAY and MF inhibition from 0. 01 to 10 μ m. We discovered that at ≥1 μ m, there was a dose-dependent suppression of αSyn 3K-GFP mRNA and protein (Fig. 3A–C), 1 μ m produced a ∼50% reduction and 10 μ m a ∼90% reduction.
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